Journal: Molecular Medicine Reports
Article Title: Asprosin activates multiple placental pathways in vitro : Evidence for potential involvement in angiogenesis, fatty acid metabolism and the mTOR, NOTCH and WNT signalling pathways
doi: 10.3892/mmr.2025.13674
Figure Lengend Snippet: DEGs in BeWo and JEG-3 cells treated with Asp. (A) A Venn diagram displaying the DEGs for Asp-treated BeWo and JEG-3 cells when compared with their respective UNT controls. (B and C) Geneset signature UMAP displaying up- and down-regulated genes in different samples. UMAPs display genes clustered by relative log-expression which were up- (red) or down- (blue) regulated in (B) BeWo and (C) JEG-3 cells, UNT control and 10 nM Asp-treated samples. (D) Volcano plots for BeWo and JEG-3 cells, indicating the most up- and down-regulated DEGs. RNA sequencing validation for (E) BeWo and (F) JEG-3 cells, using reverse transcription-quantitative PCR. *P<0.05, **P<0.001. DEGs, differentially expressed genes; UMAP, uniform manifold approximation and projection; UNT, untreated; Asp, asprosin; ZNF395, Zinc Finger Protein 395; DDIT4, DNA Damage Inducible Transcript 4; SLC2A1, solute carrier family 2 member 1; Non-ST, non-syncytialised; ns, not significant.
Article Snippet: DDIT4 , DNA damage inducible transcript 4 , 68 , Hs01111686_g1.
Techniques: Expressing, Control, RNA Sequencing, Biomarker Discovery, Reverse Transcription, Real-time Polymerase Chain Reaction